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human stem cell factor (hscf, #300-07)  (PeproTech)


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    PeproTech human stem cell factor (hscf, #300-07)
    Human Stem Cell Factor (Hscf, #300 07), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+stem+cell+factor+(hscf/human+stem+cell+factor+hscf/pm39969525-498-18-26
    Average 90 stars, based on 1 article reviews
    human stem cell factor (hscf, #300-07) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling
    Article Snippet: The efficient induction of embryonic stem cells into hematopoietic stem cells (HSCs) requires understanding the pro or inhibitory regulatory networks, which are involved in these pathways.. Transforming growth factor b family is a super family of growth factors such as TGF-b1, TGF-b2, TGF-b3, and some other cytokines.. Previous works indicate that some of TGF-b subfamilies have negative regulatory effect during hematopoietic differentiation and HSC proliferation.

    Article Title: Rapid and Sensitive Assessment of Globin Chains for Gene and Cell Therapy of Hemoglobinopathies
    Article Snippet: Human umbilical cord blood–derived erythroid progenitor-2 (HUDEP-2) cells were maintained in SFEM II, supplemented with 100 ng/mL of human stem cell factor (hSCF; PeproTech), 10 – M dexamethasone (Sigma–Aldrich), 5 μg/mL of doxycycline (Clontech Laboratories), 3 IU/mL erythropoietin (EPO; Binocrit 4,000; Sandoz GmbH), and 2 × penicillin/streptomycin (Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Paracrine effects of oocyte secreted factors and stem cell factor on porcine granulosa and theca cells in vitro
    Article Snippet: .. Granulosa and theca cells were cultured either individually or in co-culture as before with the addition of 0–100 ng/ml human stem cell factor (hSCF – Peprotech EC ltd., London, UK) with/without 5 oocytes per well. ..



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    human stem cell factor (hscf  (PeproTech)
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    ( A ) HE population (cluster 75 in integrated dataset) used for differential gene expression analysis. ( B,C ) Gene ontology (GO) terms significantly upregulated (B) or downregulated (C) in iPSC-derived vs. HSC-competent <t>human</t> embryo HE (Metascape, q < 0.05). ( D ) Arterial endothelium (AE; cluster 36, red) and HE (cluster 75, blue) populations used for differential expression analysis. ( E,F ) GO terms significantly upregulated (E) or downregulated (F) during AE-to-HE transition (Metascape batch mode, q < 0.05). ( G ) HE (cluster 75, red) and hematopoietic <t>stem</t> and progenitor <t>cell</t> (HSPC; cluster 74, blue) populations used for differential expression analysis. ( H,I ) GO terms significantly upregulated (H) or downregulated (I) during HE-to-HSPC transition (Metascape batch mode, q < 0.05). ( J ) Expression of representative endothelial identity genes FLT1 and KDR in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). ( K ) Expression of HSC genes HLF , HOXA9 , MECOM , and SPINK2 in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). For (J, K) *q < 0.05, **q < 0.005, and ***q < 0.0005 using a linear regression fit to a mixed-negative binomial model accounting for batch effects.
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    ( A ) HE population (cluster 75 in integrated dataset) used for differential gene expression analysis. ( B,C ) Gene ontology (GO) terms significantly upregulated (B) or downregulated (C) in iPSC-derived vs. HSC-competent <t>human</t> embryo HE (Metascape, q < 0.05). ( D ) Arterial endothelium (AE; cluster 36, red) and HE (cluster 75, blue) populations used for differential expression analysis. ( E,F ) GO terms significantly upregulated (E) or downregulated (F) during AE-to-HE transition (Metascape batch mode, q < 0.05). ( G ) HE (cluster 75, red) and hematopoietic <t>stem</t> and progenitor <t>cell</t> (HSPC; cluster 74, blue) populations used for differential expression analysis. ( H,I ) GO terms significantly upregulated (H) or downregulated (I) during HE-to-HSPC transition (Metascape batch mode, q < 0.05). ( J ) Expression of representative endothelial identity genes FLT1 and KDR in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). ( K ) Expression of HSC genes HLF , HOXA9 , MECOM , and SPINK2 in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). For (J, K) *q < 0.05, **q < 0.005, and ***q < 0.0005 using a linear regression fit to a mixed-negative binomial model accounting for batch effects.
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    https://www.bioz.com/product/human+stem+cell+factor+(hscf/human+stem+cell+factor/10__1016_slash_j__omtm__2024__101323-294-13-29
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    Image Search Results


    ( A ) HE population (cluster 75 in integrated dataset) used for differential gene expression analysis. ( B,C ) Gene ontology (GO) terms significantly upregulated (B) or downregulated (C) in iPSC-derived vs. HSC-competent human embryo HE (Metascape, q < 0.05). ( D ) Arterial endothelium (AE; cluster 36, red) and HE (cluster 75, blue) populations used for differential expression analysis. ( E,F ) GO terms significantly upregulated (E) or downregulated (F) during AE-to-HE transition (Metascape batch mode, q < 0.05). ( G ) HE (cluster 75, red) and hematopoietic stem and progenitor cell (HSPC; cluster 74, blue) populations used for differential expression analysis. ( H,I ) GO terms significantly upregulated (H) or downregulated (I) during HE-to-HSPC transition (Metascape batch mode, q < 0.05). ( J ) Expression of representative endothelial identity genes FLT1 and KDR in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). ( K ) Expression of HSC genes HLF , HOXA9 , MECOM , and SPINK2 in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). For (J, K) *q < 0.05, **q < 0.005, and ***q < 0.0005 using a linear regression fit to a mixed-negative binomial model accounting for batch effects.

    Journal: bioRxiv

    Article Title: Developmental regulation of endothelial-to-hematopoietic transition from induced pluripotent stem cells

    doi: 10.1101/2024.09.24.612755

    Figure Lengend Snippet: ( A ) HE population (cluster 75 in integrated dataset) used for differential gene expression analysis. ( B,C ) Gene ontology (GO) terms significantly upregulated (B) or downregulated (C) in iPSC-derived vs. HSC-competent human embryo HE (Metascape, q < 0.05). ( D ) Arterial endothelium (AE; cluster 36, red) and HE (cluster 75, blue) populations used for differential expression analysis. ( E,F ) GO terms significantly upregulated (E) or downregulated (F) during AE-to-HE transition (Metascape batch mode, q < 0.05). ( G ) HE (cluster 75, red) and hematopoietic stem and progenitor cell (HSPC; cluster 74, blue) populations used for differential expression analysis. ( H,I ) GO terms significantly upregulated (H) or downregulated (I) during HE-to-HSPC transition (Metascape batch mode, q < 0.05). ( J ) Expression of representative endothelial identity genes FLT1 and KDR in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). ( K ) Expression of HSC genes HLF , HOXA9 , MECOM , and SPINK2 in HSC-competent human embryos (CS14-17; red) and day 8 iPS (blue) AE (cluster 36), HE (cluster 75) and HSPCs (cluster 74). For (J, K) *q < 0.05, **q < 0.005, and ***q < 0.0005 using a linear regression fit to a mixed-negative binomial model accounting for batch effects.

    Article Snippet: They were resuspended in EB culture media supplemented with final concentrations of 100 ng/mL human stem cell factor (hSCF, Peprotech #300-07), 100 ng/mL fms-like tyrosine kinase 3 ligand (hFLT3L, Peprotech #300-19), 50 ng/mL bone morphogenic protein 4 (hBMP4, R&D Systems #314-BP), 10 ng/mL granulocyte colony-stimulating factor (hG-CSF, Peprotech #300-23), 25 ng/mL interleukin-6 (hIL-6, Peprotech #200-06) and 10 ng/mL interleukin-3 (hIL-3, Peprotech #200-03) and again put on a shaker at 37°C.

    Techniques: Expressing, Derivative Assay